FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852551

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852551
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19213878
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATATAG896750.0111.14065145
GTACGTA62550.097.651911
AAGCGTA72100.094.9794169
CGATTGA43550.094.949591
GTACGAT49050.093.784729
GTCGAAT57350.093.317387
ACGTATC51800.092.369583
CTGATAG142750.091.557631
CAAGCGT76150.090.1107568
GTTATTC163350.089.809268
CGTTAGA59450.088.03261
TCGTAGG66100.087.808038
CGTAGCG72000.086.875811
GTTTGAC151100.086.404828
TCTATTG194000.085.799841
TTGACTT285900.085.384811
TACGGGC62100.085.064651
TAGTACG54600.084.124197
GGTTACG50550.083.980477
CGCCAAG108800.083.278841
Produced by FastQC (version 0.11.5)