FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852552

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852552
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8627489
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCTACGTAGTAGTAGTGCGTATACTTTCTTATATNGGGACACCAGCTC430690.4992066637233615No Hit
ATTCTACGTAGTAGTAGTGCGTATACTTTCTTATATNGNGACACCAGCTC201630.23370647009807838No Hit
TTCGTCCTTGATTAATGAAAACATTCTTGGCAAATGNTTTCGCTCTGGTC196070.22726195304334784No Hit
GGGAATGAGGCACATGCAGGCATAATTTTCTTATATNGGGTCCCCCAGTA194140.22502491744701153No Hit
ACCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCNGAACCCAAAGACT177860.2061550006033042No Hit
AGGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTNGCGGCGCAATACG163240.1892091661896063No Hit
TCTCATATCGCATGGCCTCAGCCACGTTTCTTATATNGGAAAACATCCTG141000.16343109797068417No Hit
AGGCCGTCCCTCTTAATCATGGCCTCAGTTCCGAAANCCAACAAAATAGA128970.14948729578212155No Hit
ACGATACTCCGAAGAGATGCACTGACTTTCTTATATNGGGGATGTACCAG127650.14795730252452366No Hit
AACTCTTGTTCCACGGAAAGTCTATCTTTCTTATATNGGAGTTGCTGCTG122680.14219664609250732No Hit
GTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCNCCACCAACTAAGA108860.12617808031977787No Hit
GCAGTTACAGTCCTTCGTTGCCACGATTTCTTATATNGGCTCCACTCTGA98160.11377586224682523No Hit
CGTTCTGATTAATGAAAACATTCTTGGCAAATGCTTNCGCTCTGGTCCGT91770.10636930397708998No Hit
TTCGTCCTTGATTAATGAAAACATTCTTGGCAAATGNTNTCGCTCTGGTC91620.10619544110690839No Hit
AAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTNAATTCCTTTAAGT91440.1059868056626905No Hit
GTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGANAACATTCTTGGCA90110.1044452215470805No Hit
GGGAATGAGGCACATGCAGGCATAATTTTCTTATATNGNGTCCCCCAGTA89960.10427135867689893No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAG117800.0134.870835
CTACGTA121300.0134.219484
TTCTACG126400.0133.743582
TCTACGT133350.0128.631873
ATTCTAC141500.0127.483861
CGTAGTA124050.0123.642347
ACGTAGT121100.0123.495086
ACGATAC53600.0122.535261
TTGCGTC91800.0118.5571
AATGAGG87500.0117.3858644
AACGTTG45600.0116.575981
TTAGGCA58500.0115.380321
TACGGGC51200.0114.481251
TACGTGA20550.0114.1236045
AGCTAGT52850.0114.031628
CATATCG47200.0112.862924
ACGTATG18500.0112.475226
TACCTTA30350.0112.466041
ATCGACG32550.0112.0295649
CGATACT51550.0112.014982
Produced by FastQC (version 0.11.5)