FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852553

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852553
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15926759
Sequences flagged as poor quality0
Sequence length151
%GC49

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCTAA184350.0184.4190535-39
GGCTAAA219150.0164.5845335-39
CTTATAT7377700.0143.8485130-34
TATATCG89100.0127.48897630-34
CGTATAT142700.0111.9322530-34
GATATAG618100.0108.10476145
CGATACT76950.0103.4916842
CTTAGAT185250.0101.62710630-34
GACGTAA68750.0100.015085
TTATACG33150.099.9532530-34
TTATATG812900.099.1522530-34
ACGATAC85150.098.3492661
ACGCGTA43650.097.987264
GCCGTAA53150.097.316295
TACCTTA142800.095.483481
TATATAG140300.094.46757530-34
GGACGTA77800.093.8189244
TGTACCG69700.092.5900347
TTACGCG40750.092.145139
GTACGAT41600.092.064349
Produced by FastQC (version 0.11.5)