FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852561

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852561
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19027887
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC5245722.756858919752887No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC3843342.0198459240377034No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC3656551.9216794802281514No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC2720321.42964901988329No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG1127680.5926459411914733No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCGAGCGTG1064650.5595208758597316No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCTGG912480.47954878016671No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGAGTTCC527000.27696191384781715No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAG504190.2649742454325065No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCTG351200.18457120330807095No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGCTTC296550.15585020028760943No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTA285720.15015855412637252No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT268010.14085116229668593No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTGC256170.13462871626260972No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATGGGGTTTG238030.125095340328645No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTTTA228850.12027084247452173No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATACGGATGA224030.11773771832889275No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGCTGG213700.1123088443819327No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTT207850.10923440947489334No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTCGGATTT205590.10804667906636192No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCT192290.10105693816659728No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATG1196000.0127.988051
AGTCTCT1580350.0125.433671
GTCTCTC1680400.0118.1873252
AGCTGGT1798200.0110.910259
TCAGCTG1806500.0110.056857
TCTCTCA1833750.0108.4279253
CAGCTGG1899250.0104.922878
TCTCAGC1943850.0102.222915
CTCTCAG1977150.0100.787124
CTCAGCT2027100.098.490976
CTGATGG2009150.075.8102652
GGCTCAA2052550.074.2118457
GATGGCT2065850.073.541884
GCTCAAA2078750.073.430088
CTCAAAC2083600.073.400799
TGATGGC2105250.072.386043
ATGGCTC2201250.068.991945
TGGCTCA2242900.068.062266
TCAAACA2138800.053.468019
GAGGTTA277650.050.217392145
Produced by FastQC (version 0.11.5)