FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852562

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852562
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15576240
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC3366102.16104785237002No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC3332392.1394059156766976No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC1336680.8581531871619853No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC1304120.8372495544495977No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCTG472350.303250335125807No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAG440240.2826356039711766No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTTTA342590.21994396593786433No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT339900.21821697662593795No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAAAG285330.18318284772191493No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGAGTTCC261740.16803798606082085No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG253030.16244613590956483No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTA247250.15873535590103902No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGGGTG237640.1525657026342686No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTT212800.1366183366460712No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCATAGTTA212450.13639363543448227No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTGC186150.11950894439222816No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC182560.1172041519647874No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATG660200.0123.141261
AGTCTCT1044600.0116.145491
GTCTCTC1061950.0114.157292
TCTCTCA1071750.0113.217993
AGCTGGT1128500.0107.827899
TCAGCTG1140750.0106.4289257
CAGCTGG1156500.0105.186618
CTCTCAG1256000.096.828644
TCTCAGC1291250.093.9661255
CTCAGCT1300300.093.74886
GGCTCAA1316450.062.2847636
CTCAAAC1327500.062.2796368
CTGATGG1330500.061.9161951
GCTCAAA1338300.061.5061077
TCAAACA1371250.060.3344849
GATGGCT1361500.060.0081373
ATGGCTC1425250.057.3952184
TGGCTCA1448750.056.519065
CTTCCGA43000.054.43574
CCGATCT43400.053.4308247
Produced by FastQC (version 0.11.5)