FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852565

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852565
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15508893
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC3288312.120273832568192No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC2480801.5995983723660998No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC2227481.4362598284738954No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC1703071.098124798462405No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGAGTTCC550350.35486091753937565No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCTGG434830.2803746211931438No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG418670.2699547930339064No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCTG330000.212781144340863No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAG328280.21167210322490457No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCGAGCGTG310470.20018836934396286No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGCTTC286470.1847133770282637No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATGGGGTTTG271470.17504150683095177No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGCTGG263730.1700508218091388No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTTTA259420.16727177110577782No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATACGGATGA226360.14595496919090228No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT222710.14360148077622303No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTGC206440.133110725568872No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTA186060.11996987792745749No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCT157360.10146436628326728No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCTCT1127700.0123.7254641
TCTGATG782650.0123.359151
GTCTCTC1167450.0119.271672
TCAGCTG1203400.0115.834827
AGCTGGT1223500.0114.543629
TCTCTCA1250100.0111.504743
CAGCTGG1312250.0106.7429358
CTCTCAG1453200.096.336214
CTCAGCT1464400.095.521566
TCTCAGC1482950.094.4486165
CTGATGG1305800.073.8095552
CTCAAAC1338100.072.0057459
GGCTCAA1340600.071.464977
GATGGCT1343900.071.379314
GCTCAAA1365550.070.362788
TGGCTCA1444050.066.2150046
TGATGGC1464900.065.7845463
ATGGCTC1498250.064.048385
TCCGATC30600.058.9890986
CTTCCGA33600.054.604244
Produced by FastQC (version 0.11.5)