FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852572

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852572
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8041300
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGGC17000.049.475235
TGATACG19450.045.1101463
TACGGCG18750.043.695266
GATACGG21650.039.520934
GGCTCAA85350.031.1759476
GCTCAAA88500.030.0616237
GTCTCTC72700.029.5235422
CTCAAAC91550.029.3759338
TCTGATG79400.028.9124341
GATGGCT94950.027.9508193
AGTCTCT82100.025.8244081
ATGATAC35050.025.6533932
TCAAACA104350.025.4249069
CTGATGG107950.025.1261981
TGATGGC108900.025.0363582
ATGGCTC109950.024.5330054
TCTCTCA87350.024.0737383
CTCTCAG106450.020.7757744
AGCTGGT113850.019.1647959
TCAGCTG120650.018.1455127
Produced by FastQC (version 0.11.5)