FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852574

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852574
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14607369
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC1298240.8887569007122365No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC824540.5644685227024798No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC630010.43129601230721293No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC398220.2726158283534838No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCTCT285250.093.511171
GTCTCTC307600.086.916462
TCTGATG214900.086.059611
GGCTCAA489250.074.877866
TCTCTCA360650.074.1113363
GCTCAAA494650.074.0523157
CTCAAAC500200.073.403078
TCAAACA515650.071.061969
GATGGCT517100.070.858183
CTGATGG555850.066.662241
CTCTCAG408350.065.807624
TCTCAGC415250.064.655335
TGATGGC583000.063.333732
ATGGCTC597150.061.454864
TCAGCTG455050.059.2135127
CTCAGCT490550.055.2595256
AGCTGGT494300.054.7149059
CAGCTGG519850.052.2497378
TGGCTCA730900.050.3825875
TGATACG27250.044.9713023
Produced by FastQC (version 0.11.5)