FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852577

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852577
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14448397
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC614190.42509213997926554No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC405900.28093081883062876No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC379910.26294266415852224No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC249110.1724135902411873No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGCTGG229420.15878578087243866No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCTCT230050.089.427351
GTCTCTC237200.087.12752
TCTGATG156200.076.010491
TCTCTCA289450.071.499913
CTCTCAG317200.065.586124
TCTCAGC318850.065.353645
GGCTCAA282750.062.975656
GCTCAAA290400.061.3581247
CTCAAAC293850.060.883388
GATGGCT300650.059.1886563
TCAAACA308950.057.812449
TCAGCTG383950.054.2623867
CTCAGCT390800.053.596936
AGCTGGT411600.050.7559979
CAGCTGG415700.050.396128
CTGATGG357850.050.1759761
TGATACG21200.049.9392553
TGATGGC391450.045.8113332
ATGGCTC394550.045.468674
TACGGCG20300.045.0009236
Produced by FastQC (version 0.11.5)