FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852578

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852578
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5356927
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC1343452.5078743839518443No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC895131.67097666255299No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC768641.4348524816559942No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATGGGGTTTG528930.9873757846616167No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC506490.9454860967864598No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATGGGGTTTGA162640.3036069000006907No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC155290.2898863471538813No Hit
CTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATGGGGTTTGACC147240.27485907498832823No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT135690.25329820622905636No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTC104790.19561588201593935No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCT89980.16796943471508946No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCTG79370.14816330332670205No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGTTTC69790.13027991607875186No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG61400.11461795167266606No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATG187900.0120.450651
AGTCTCT265950.0110.4179151
GTCTCTC311200.094.582012
TCTCTCA320850.092.032263
TCAGCTG382050.077.572227
CTGATGG520750.077.54821
CTCAAAC529650.075.8839348
GGCTCAA528800.075.169626
TCAAACA540650.074.349
GCTCAAA539900.073.97337
AGCTGGT404550.072.684419
ATGGCTC546950.072.664654
GATGGCT550550.072.254663
CAGCTGG413200.071.180378
TGGCTCA574950.069.427225
CTCTCAG429750.068.897214
CTCAGCT437150.067.993746
TCTCAGC438950.067.5510255
TGATGGC662000.061.1669042
GAACTAA100100.057.505592145
Produced by FastQC (version 0.11.5)