FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852579

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852579
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9303924
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC3517283.7804264093300852No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC2058382.21237834702863No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC1982292.1305956497495036No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC1131451.2160997875735011No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC453540.48747173773130564No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT361880.38895416600565524No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCTG265040.2848690509509751No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTC255920.27506673528287634No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCT206400.2218418809096033No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTTTA178890.19227371160813436No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATACGGATGA149580.16077087474059332No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG146270.1572132360496496No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTA132250.14214432534057675No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCTGG122630.13180460201523572No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTGC104760.1125976523453975No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATG451700.0126.1697851
AGTCTCT539100.0106.850731
GTCTCTC582600.098.858422
TCTCTCA604150.095.073713
TCAGCTG705800.081.3623057
CTGATGG1204450.081.270391
AGCTGGT731450.078.132519
CAGCTGG741900.077.178568
CTCAAAC1305150.075.0224468
GGCTCAA1297350.074.81376
GCTCAAA1309950.074.155367
TCAAACA1328750.073.717259
GATGGCT1331400.072.752173
TGGCTCA1347550.072.304885
ATGGCTC1359400.071.446044
TGATGGC1389550.070.582132
CTCTCAG845850.068.2069244
CTCAGCT869700.066.537256
TCTCAGC871350.066.168545
ACCCCTC155250.064.3951145
Produced by FastQC (version 0.11.5)