FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852594

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852594
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14108151
Sequences flagged as poor quality0
Sequence length161
%GC52

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCTCGGTTCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGT1575581.1167870261666464No Hit
AGATCTCGGTAGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGAT1485261.0527672974296916No Hit
AGATCTCGGTCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTT1415131.0030584447246134No Hit
AGATCTCGGTAGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGAT1402340.993992763474108No Hit
AGATCTCGGTTCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCT871730.6178910333466093No Hit
AGATCTCGGTCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTT829910.5882485947308049No Hit
AGATCTCGGCCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTT493200.3495851440773493No Hit
AGATCTCGCCCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTT491710.34852901702001915No Hit
AGATCTCGGCTCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGT332420.23562265530047136No Hit
AGATCTCGGCAGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGAT308700.21880967959585917No Hit
AGATCTCGCCAGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGAT297140.2106158347752303No Hit
AGATCTCGCCCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTT280920.1991189348625486No Hit
AGATCTCGGCCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTT273450.19382412337378582No Hit
AGATCTCGGCAGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGAT272460.19312240136925102No Hit
AGATCTCGCCTCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGT258640.18332664570998708No Hit
AGATCTCGCCAGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGAT250670.17767742916842894No Hit
AGATCTCGGTGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATA243590.17265905362084655No Hit
AGATCTCGGTGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATA229090.16238130708978094No Hit
AGATCTCGGCTCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCT177560.12585632234869049No Hit
AGATCTCGGTTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTT162360.11508240874371135No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGC1251000.0153.188743
TCGGTCT1297300.0152.381276
CTCGGTT1605300.0152.356615
TCGGTAA404350.0151.426456
TCGGTAT423200.0150.968986
CTCGGTC2893850.0150.850115
CGGTAGT519450.0150.449467
TCGGTTC657250.0150.050026
CTCGGTA2067450.0149.112155
TCGGTTA241050.0148.856086
TCGGTCC728900.0147.89536
TCTCGGC1353250.0146.712974
TCTCGCC948500.0146.363344
TCGGTGT849450.0145.673426
TCGGTGC711150.0145.427386
CGGTCTG662850.0144.439547
GATCTCG12988850.0143.533742
AGATCTC12975900.0143.497471
TCGGTGA640300.0143.365986
CGGTCAA167550.0142.925147
Produced by FastQC (version 0.11.5)