FastQCFastQC Report
Wed 11 May 2022
EGAF00004852600

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852600
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16088365
Sequences flagged as poor quality0
Sequence length161
%GC50

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCTCGGTAGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGAT725210.450766749759842No Hit
AGATCTCGGTTCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGT474350.29484040174374465No Hit
AGATCTCGGTCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTT407290.25315810525183885No Hit
AGATCTCGCCAGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGAT350170.21765418673681258No Hit
AGATCTCGGTTCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCT324690.2018166544580509No Hit
AGATCTCGCCCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTT306400.19044819035371213No Hit
AGATCTCGGTCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTT287100.17845194337647113No Hit
AGATCTCGGCAGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGAT266770.16581548218231001No Hit
AGATCTCGGCCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTT220060.1367820782285832No Hit
AGATCTCGCCCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTT215970.13423986837692953No Hit
AGATCTCGCCTCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGT188920.11742647559276534No Hit
AGATCTCGGCTCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGT176650.10979984603780434No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)