FastQCFastQC Report
Fri 1 Jul 2022
EGAF00004852603

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852603
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19045285
Sequences flagged as poor quality0
Sequence length161
%GC52

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCTCGGTAGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGAT2044611.0735518003537359No Hit
AGATCTCGGTTCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGT1283680.6740145920630749No Hit
AGATCTCGGTCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTT1151160.6044330657167903No Hit
AGATCTCGGTTCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCT684870.3596008145848172No Hit
AGATCTCGGTCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTT644390.33834621009871996No Hit
AGATCTCGCCCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTT455350.23908804725159008No Hit
AGATCTCGGCAGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGAT407640.21403722758677543No Hit
AGATCTCGGTGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATA404080.21216799853612062No Hit
AGATCTCGCCAGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGAT401540.21083433511233882No Hit
AGATCTCGGCCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTT392490.20608250283469112No Hit
AGATCTCGGCTCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGT268860.1411687984716427No Hit
AGATCTCGCCTCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGT246010.12917107830100732No Hit
AGATCTCGCCCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTT217980.11445352484880116No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)