FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852612

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852612
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17304438
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC4824022.7877357242113265No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC4135442.3898146822219823No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC2412881.3943706233048425No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC2012111.1627710764140389No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT355020.20516124245121398No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTGC300990.17393803832288574No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC299810.17325613232859685No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAG291350.1683672130814072No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCTG280030.1618255386277208No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTT258050.14912359476800113No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGGAGA232640.13443950043335703No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG222660.1286721938036936No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCAATC216760.12526266383224927No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGATGG201480.11643255909264433No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGCTTT190720.11021450104302723No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCT181730.10501930198484344No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATG987150.0118.082231
AGTCTCT940500.0114.8777541
GTCTCTC1016200.0105.890822
TCTCTCA1087600.098.825983
CAGCTGG1210450.090.278728
AGCTGGT1230600.089.194899
TCAGCTG1222500.088.534747
CTCTCAG1281950.084.652944
CTCAGCT1284000.084.519996
TCTCAGC1360200.079.5556955
GGCTCAA1972700.070.87616
CTCAAAC2001950.070.640978
GCTCAAA1997550.070.230267
CTGATGG2008800.070.065181
TCAAACA2029200.069.6278469
GATGGCT2044600.068.146143
ATGGCTC2132900.065.46944
TGATGGC2202300.063.9124372
TGGCTCA2203950.063.6135445
TGATACG14200.038.7930343
Produced by FastQC (version 0.11.5)