FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852624

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852624
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5221689
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC568891.0894750721461963No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC373210.7147304253470477No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC259400.49677412806469323No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC163090.31233189107968706No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC107280.20545076506854393No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTC71000.1359713303492414No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT61410.11760562530629458No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCTCT143950.099.78941
GTCTCTC154100.092.7764362
TCTGATG89250.092.635371
TCTCTCA170050.083.560373
CTGATGG238600.075.804591
GGCTCAA245450.073.812236
GATGGCT245750.073.160913
CTCAAAC249050.072.567158
GCTCAAA249950.072.276857
TGATGGC254550.071.060932
TGATACG7300.070.510933
TCAAACA256950.070.13999
CTCTCAG206900.069.411674
TCTCAGC215950.066.232945
CTCAGCT229450.062.750376
TGGCTCA291750.062.144545
ATACGGC8700.059.1615035
CAGCTGG251600.057.5114338
AGCTGGT251750.057.363089
TACGGCG9450.054.469286
Produced by FastQC (version 0.11.5)