FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852625

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852625
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13611725
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC1084260.7965632570449374No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC751830.5523399862985772No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC726240.5335400178889891No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC492860.36208489372214026No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATGGGGTTTG204580.15029689477270514No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC157700.11585599914779353No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTATG153320.11263818509410085No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCTCT321250.091.54581
TCTGATG260000.085.765531
GTCTCTC357450.082.124462
TCTCTCA397600.073.863853
GGCTCAA535450.063.5272376
CTCTCAG465800.063.282554
GCTCAAA544650.062.4658627
GATGGCT548650.061.892443
CTCAAAC554950.061.6585168
CTGATGG567550.060.738411
TCTCAGC504450.058.7642825
TCAAACA582600.058.595789
CTCAGCT527450.056.3675776
TGATGGC611350.056.034042
ATGGCTC616300.055.239794
TCAGCTG540150.054.7992637
AGCTGGT556250.053.3823979
CAGCTGG587150.050.5603148
TGGCTCA681300.050.2141465
TCCGATC56350.046.573146
Produced by FastQC (version 0.11.5)