FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852638

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852638
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47570163
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG558290.1173613804939033No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG26550.022.8764616
TCACGCT262450.019.6965378
TCGACGA32500.019.1034667
CCGGCTT294450.018.88522
GCCGGCT287700.018.2370151
TTCACGC286450.018.10917
CCCGTAT25050.017.8888741
ACGCTCA297850.017.71808810
AACGCTA284800.017.6568423
CGCTACT77950.017.60802525
ACGCTAC286350.017.54641724
CGGCTTC322000.017.4441223
CACGCTC301800.017.031529
GAAAACG260600.016.86734220
CGAACGA38800.016.81822232
CGGTATA52200.016.5053520
AAAACGC307300.016.4494421
CGCTACC224150.016.322225
CGACGAG37150.015.7434888
AAACGCT324300.015.60335522
Produced by FastQC (version 0.11.5)