FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852639

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852639
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47570163
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA479460.10079006876642403No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG146950.013.46590545
GGCATAG148000.013.10855835
ATAGGGG161250.012.07433338
GGGAGGT239950.011.7675793
GTTGTCG23050.011.71022726
GGAGGTC226350.011.640564
CATAGGG171950.011.3360737
GTCACTC223500.011.29600058
GAGCTTA178800.011.17281444
CACCGAA256900.011.04136445
GGTCACT229500.010.9222437
GCACCGA263100.010.79939944
GAGGTCA254150.010.67740255
GCATAGG183000.010.63859836
TGGCATA189850.010.51224334
TAGCGTA8100.010.28166737
ACTCTCC247450.010.26603111
AGGTCAC271850.09.9075946
ACCCGGC70550.09.79151255
TAATCGG43500.09.7761994
Produced by FastQC (version 0.11.5)