FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852645

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852645
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52365294
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN656610.12539030144660318No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG137700.012.74707245
GGCATAG142100.012.19266435
ACCCGGC83000.011.9516235
ATAGGGG149100.011.93746338
CATAGGG150250.011.59117937
GAGCTTA164150.010.95347544
GCATAGG162900.010.801846536
TCACCCG103100.010.4942843
GTCACTC217650.010.4379548
ATAGCTC93600.010.36141739
TGGCATA172450.010.1383534
GGGAGGT261550.010.0537063
CGGACAC99700.010.018887513
GGTCACT233250.09.7201847
ACTCTCC238050.09.66682411
GGAGGTC245600.09.6349084
CACCCGG110550.09.583464
AGGTCAC250500.09.4457886
TCAGACG91800.09.3598132
GAGGTCA263100.09.1224175
Produced by FastQC (version 0.11.5)