FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852648

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852648
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48061511
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1772050.3687045960748092No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG942510.1961049455977362No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT508790.10586225639056582No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT369600.024.65736845
ACCGCGT387050.023.6878366
AGACCGC389950.023.5926234
GACCGCG389650.023.5181795
CAGACCG401450.022.9501973
CCGCGTT399900.022.9153777
CGCGTTC402900.022.7613838
TCAGACC434000.021.7021772
AGAACGA430900.021.13393244
GCGTTCT442100.020.7788059
ACGCTAC261950.020.27015724
AACGCTA261900.020.21397623
AATACGG455200.019.93023932
ATACGGA461250.019.71272733
TACGGAG458200.019.68189234
TCACGCT278300.019.5885338
CGCTACC274650.019.25089525
CTCAGAC533450.019.2430021
CAATACG479550.019.03552631
AAAACGC277650.019.01865621
Produced by FastQC (version 0.11.5)