FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852651

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852651
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55183162
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG724680.13132266686711427No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG252550.015.28162545
GGGAGGT355450.014.5643083
ATAGGGG269350.014.45161438
GGCATAG268150.014.30048535
GGAGGTC351100.014.2894254
GTCACTC357250.013.81502158
GGTCACT372550.013.2668517
GAGCTTA300350.013.03796544
GTTGTCG30750.012.73776626
TGGCATA306950.012.5736534
ACTCTCC391650.012.52262311
CATAGGG319550.012.09097537
GAGGTCA428800.012.0811465
AGGTCAC426450.011.9241496
GCATAGG335250.011.5381736
TCACTCT446900.011.294589
CACTCTC441100.011.12664410
TGGGAGG386050.010.7937812
TAGGGGA463700.010.63595539
CTGGGAG461500.010.0480661
Produced by FastQC (version 0.11.5)