FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852652

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852652
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53790138
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT2710400.503884187841273No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1051990.19557302492884476No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG756300.1406019817238617No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA730750.13585204038703155No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT472550.027.7539345
AGAACGA560950.023.43233544
AGACCGC589350.022.9102654
CAGACCG598400.022.6616213
GACCGCG599500.022.481055
ACCGCGT601350.022.4270216
CCGCGTT615350.021.916417
CGCGTTC616200.021.8971148
TCAGACC650300.021.0764062
GCGTTCT663500.020.4036189
AAGAACG645600.020.37108243
AATACGG661850.020.24858532
ATACGGA666800.020.07744233
TACGGAG666400.020.045634
CAATACG677150.019.83397531
CTCAGAC726850.019.6594091
ACGGAGA699150.019.13529435
AACGCTA309000.018.98283623
CGTTCTC723250.018.95761510
ACGCTAC310400.018.91169524
Produced by FastQC (version 0.11.5)