FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852653

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852653
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53790138
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA651660.12114860162656582No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG616930.11469202774679627No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG609220.11325867949994847No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG568800.10574429089585156No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG45550.016.3492726
AGCTTAG220500.014.21362945
TGTCGTT57000.013.2990628
GGGAGGT363750.013.295893
GGCATAG237350.013.062235
ATAGGGG241550.012.98386638
ATATAGC67500.012.8678186
GGTCACT358250.012.8512117
GTCACTC362050.012.7913858
GCTAAGT60900.012.48725814
GGAGGTC378100.012.4459874
ACTCTCC383650.012.07566411
GAGCTTA259100.012.06139844
TGGCATA266150.011.70766734
AGGTCAC407500.011.6513496
TCGTTCC66350.011.4252230
GAGGTCA417900.011.4005665
GTCGTTC67650.011.07216629
CGTTCCC69900.011.00593531
CACTCTC420650.010.93421110
Produced by FastQC (version 0.11.5)