FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852778

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852778
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49328544
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG499370.10123347650398924No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCTA152600.019.65544923
ACGCTAC134350.019.5284724
CGCTACC139600.018.84267625
TCACGCT182850.017.0397438
ATGCGCG31450.016.95636744
AAAACGC181550.016.66989721
TGCGCGT33150.015.97897145
TTCACGC198850.015.9628227
CCCGTAT29500.015.7922231
ACGGTAC75800.015.730803545
GAAAACG196700.015.40872220
TACGGTA77250.015.40856844
CCGGCTT242100.015.2408842
ACGCTCA214850.015.20243410
GTCCGTT39500.015.1558211
GCCGGCT237850.015.12058451
GTTAGTT118450.014.81714944
AAACGCT205850.014.66931622
GTTTTCG207750.014.66898745
CCTTACG82900.014.41003241
Produced by FastQC (version 0.11.5)