FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852779

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852779
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49328544
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG515650.10453379690266147No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG159450.016.2421345
GGCATAG186300.014.00980435
ATAGGGG195600.013.59786538
GAGCTTA197750.013.23262944
GGGAGGT325850.013.0042053
GTCACTC304600.012.4547448
GGAGGTC320650.012.3858014
TAGCGTA11950.012.239437
TGGCATA215550.012.20262734
CATAGGG216500.012.16025237
ACTCTCC318400.012.09834511
GGTCACT316300.012.0367247
GAGGTCA337550.011.69955
GTTGTCG25900.011.55283526
AGGTCAC340150.011.5367416
GCATAGG230700.011.37218836
TCACTCT357400.010.8728889
CACCGAA157850.010.71927645
CACTCTC365150.010.51869510
GCACCGA160800.010.35448744
Produced by FastQC (version 0.11.5)