FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852781

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852781
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49790532
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN580730.11663462443020291No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGCTC79850.013.7185019
AGCTTAG106500.013.71277345
GGCATAG111600.013.02631935
GTTGTCG26900.012.79909926
ATAGGGG121050.012.75280538
GCAGCGC93800.011.6297437
GAGCTTA129200.011.37315344
CAGCGCT98900.011.3487198
TCAGACG77050.011.2372882
TGGCATA134200.011.23535134
CGCTCTC101150.010.89704111
CGTCGAT22350.010.76957812
ACCCGGC72650.010.6186145
GGGAGGT261850.010.5294183
TGTCGTT35500.010.39571728
CATAGGG146350.010.31787337
ACGGACA77750.010.21692618
CACCCGG111300.010.1845761
TCACCCG82200.010.1774183
GCGCTCT106650.010.14512410
Produced by FastQC (version 0.11.5)