FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852782

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852782
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52946465
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT2951460.5574423146096723No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1189480.22465711355800616No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG823730.1555779030762488No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA823390.155513687268829No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT546150.025.62029545
AGACCGC621600.022.7731444
GACCGCG626500.022.5662755
ACCGCGT627350.022.5572436
CAGACCG634350.022.365123
CGCGTTC642250.022.0409268
CCGCGTT643450.021.9928287
AACGCTA301850.021.86044923
ACGCTAC303750.021.7385224
AGAACGA647450.021.66779144
TCAGACC671000.021.349292
CGCTACC310050.021.20247825
GCGTTCT683300.020.743139
AAAACGC320500.020.68462421
TCACGCT336000.020.1379288
AAACGCT329950.020.13483822
AATACGG702600.019.9898932
GAAAACG331250.019.98616420
CTCAGAC755550.019.912981
ATACGGA709550.019.7904533
Produced by FastQC (version 0.11.5)