FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852783

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852783
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52946465
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG977730.18466388643698875No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA815650.1540518332999191No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG792550.14968893579580808No Hit
GTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGT569440.10755014522688153No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG553480.10453577967858667No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG295950.016.3639945
GGGAGGT429400.015.7726883
GGAGGTC441100.015.1602534
ATAGGGG333100.014.45309438
GGCATAG332850.014.42343635
GAGCTTA343500.014.05733444
ACTCTCC482600.013.77498911
TGGCATA353700.013.64971834
TGGGAGG385100.013.1518862
CACCCGG119200.013.1222611
GTTGTCG29450.012.91095426
CATAGGG390900.012.38479437
GGTCACT540100.012.3201437
CACTCTC554600.012.088119510
GTCACTC552500.012.0769958
GCATAGG411500.011.78123836
TCTCCCC571250.011.74755513
CTCCCCA429850.011.59098114
GCTCTGT578900.011.53378222
AGGGATC195450.011.4889652
Produced by FastQC (version 0.11.5)