FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852784

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852784
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47194414
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC72450.021.8911724
ACCGCGT76000.021.1350336
GACCGCG80200.020.028215
CAGACCG83400.019.3406663
CCGCGTT84600.018.8801867
GAACGAT87750.017.73699445
CGCGTTC95700.016.8548748
ATACGGA94600.016.50619333
AACGCTA72800.016.50176823
ACGCTAC73850.016.11549824
AATACGG98000.015.86564432
GTTAGTT127700.015.77308344
TCGGCGC86650.015.36271845
AGAACGA107600.014.70372944
CGCTACC80850.014.69263825
CCCGTAT28550.014.6669561
TTCGGCG94400.014.471086544
TACGGAG109600.014.3711634
CAATACG110100.014.26506831
AAGAACG114400.013.73136943
Produced by FastQC (version 0.11.5)