FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852785

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852785
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47194414
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCATAG156600.015.04034235
GTTGTCG30700.014.73256426
AGCTTAG160650.014.7000245
ATAGGGG165450.014.57389338
GTCACTC254650.014.0263338
ATATAGC39450.013.6944686
GGTCACT260700.013.6404977
GGGAGGT292100.013.2876973
GAGCTTA182950.013.08081244
CATAGGG184350.012.8601237
GGAGGTC285200.012.8504274
ACTCTCC282750.012.82059211
TGGCATA191050.012.45880634
AGGTCAC302300.012.1747626
TGTCGTT39050.012.15921628
CGTCGAT29500.012.12802912
GCATAGG198750.011.91792336
GAGGTCA315950.011.7845565
GCTAAGT40050.011.57189114
TCAGACG82450.011.5231412
Produced by FastQC (version 0.11.5)