FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852788

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852788
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44503071
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1738780.3907101152637309No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT1281320.28791720912923063No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGC1199940.26963083064537274No Hit
GTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGC558620.12552392170868387No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT509590.11450670449237087No Hit
CGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTC487860.10962389539364598No Hit
CGCTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGT487560.10955648431543073No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCTA362250.025.2942723
TCACGCT372100.025.1333248
ACGCTAC364650.025.1216224
CGCTACC370900.024.69885425
AAAACGC377450.024.26814821
AAACGCT387650.023.75218222
GAAAACG386400.023.66516720
GCCGGCT379750.023.5359021
GTTTTCG395250.022.96211645
CCGGCTT418500.022.8395542
CACGCTC414350.022.5434159
TCGGCGC275500.022.4354845
CGTGGTT409750.022.4152341
GTTAGTT284350.022.293144
TCGTGGT409750.022.28337540
TTCACGC432250.021.8336377
CGGCTTC451400.021.737973
ACGCTCA459800.021.08794210
CTACCTC440300.021.05078727
TTCGGCG307600.020.4325644
Produced by FastQC (version 0.11.5)