FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852790

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852790
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44932005
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGTT37100.017.2397021
CCCGTAT30400.016.2239231
GTCCGAT25000.015.9447811
GTCGGTT38050.015.9214621
TTCGACG19900.015.3768336
GTCCGGT31800.014.8014561
CTCCGTT66950.014.7335991
CCCGGAT85450.014.5219461
AACGCTA67450.014.24352123
ACGCTAC68400.014.11148224
TGCGCGT18700.013.71622845
GTCCGAA48400.013.6335071
CTCCGAT52900.013.5806431
CGCTACC71050.013.45850425
GTCCCGT45050.013.3975481
CTCGGTT81600.013.3855711
ACGGTAC61550.013.26936145
ATGCGCG18150.013.26412444
CCCGGTT43550.013.2384491
AGACGCT142650.013.07537641
Produced by FastQC (version 0.11.5)