FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852792

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852792
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47591170
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG672420.14129091594091928No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG43100.025.5271456
TCGACGA50000.022.2743937
CGGTATA71800.019.86714720
GTTCGAC57050.019.3266585
GCCGGCT201500.018.5798851
CCGGCTT220000.018.4923672
CGACGAG59450.018.3930878
TTCACGC215600.017.7093987
GTCCGTT38550.017.3443451
TCACGCT219750.017.2315968
CAGTTCG76000.015.8992633
CGGCTTC266500.015.8905423
ACGCTCA244600.015.59134110
CACGCTC251750.015.0234099
AGTTCGA75950.015.0209184
ATGACGG93200.014.87090216
CCCGTAT27550.014.7087821
GACGGTA98500.014.57326918
AACGCTA253400.014.51716723
ACGCTAC252250.014.51197824
Produced by FastQC (version 0.11.5)