FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852793

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852793
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47591170
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG142200.012.40359738
GTTGTCG35850.012.17351226
AGCTTAG150050.011.73924345
AACTCTA119400.011.47432845
GGCATAG153050.011.259541535
GGGAGGT194900.011.0347583
GGAGGTC194200.011.0278584
ATATAGC48100.010.8541646
GCTAAGT44450.010.47728914
GAGCTTA172150.010.31040244
TGGCATA176750.09.95345734
CATAGGG175450.09.91190937
CACCGAA231300.09.86226545
AGGGATC118500.09.8442612
GGTCACT216050.09.7282397
GCATAGG178850.09.69819136
GTCACTC219650.09.6608898
TGTCGTT44900.09.56961528
TAATCGG42850.09.4597914
GCACCGA241850.09.32954544
Produced by FastQC (version 0.11.5)