FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852794

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852794
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52853005
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG27550.018.753166
ACCGCGT98850.018.6566966
GACCGCG99200.018.4097545
AGACCGC103200.017.8050294
CCGCGTT105700.017.4263767
CAGACCG111000.016.7562353
CGCGTTC110500.016.750718
CGGTATA41950.016.70664620
TCGACGA32550.016.2175467
ACGCTAC126100.014.16180524
AACGCTA126900.014.143332523
CCGTACG62150.014.0236054
GTCCGTT41300.013.996051
GAACGAT131650.013.94016245
CGACGAG36600.013.8706118
TTCGGCG61500.013.6603944
CCCGCGT16350.013.6188341
CGTACGC63750.013.5659315
CTCCGTT76350.013.5215371
TCGGCGC63450.013.34677545
Produced by FastQC (version 0.11.5)