FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852796

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852796
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50147388
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGGTTCTTCTGCACATATTGGAAGGCATTGGTCATGTAGCCCCCTCCAC730860.14574238642299775No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG657840.1311813089846275No Hit
CCCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAGA562610.1121912870117981No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGGTT132750.030.202181
CCGGTTC169650.022.7384592
CGGTTCT187900.021.2126963
TTCGACG22700.021.1077796
GCCGGCT168650.020.6096131
CCGGCTT213500.019.3016552
TAGCCCC192800.018.85594239
TTCACGC205550.017.4773757
TCACGCT204650.017.356388
AACGCTA199900.017.354723
GCACTAC90750.017.35448325
ACGCTAC201850.017.17646224
GTCCGTT38750.016.9055841
CGCTACC206600.016.7162325
CCCGTAT34050.016.4623241
TCGACGA30050.016.3941337
ACGCTCA222100.016.0636210
CGGCTTC261150.016.0039043
AAAACGC219650.015.95664221
GCCCCCT230100.015.78955241
Produced by FastQC (version 0.11.5)