FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852797

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852797
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50147388
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG832940.1660983818339651No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG250850.015.84620445
GTTGTCG37350.015.78071726
GGGAGGT339250.015.7128593
GGAGGTC354300.014.8679964
GGCATAG266250.014.84573535
ATAGGGG272800.014.64642138
GAGCTTA288200.013.844027544
GAGGTCA389750.013.4241735
TGGCATA297150.013.4230934
GTCACTC381900.013.412628
GGTCACT386600.013.2608987
ACTCTCC392050.013.26085411
ATATAGC53600.012.847686
TGTCGTT47800.012.66019528
TGGGAGG333800.012.5169452
CATAGGG327700.012.16505637
AGGTCAC429600.012.1637086
TCACTCT428200.012.1305419
CACTCTC426250.012.07015410
GCTAAGT50900.011.84949314
Produced by FastQC (version 0.11.5)