FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852803

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852803
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49014490
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG733370.14962310125026293No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA699570.14272718128863526No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG649640.13254039774768647No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG222150.016.48571445
GGCATAG249050.014.59345235
GGGAGGT357250.014.4567763
GTTGTCG29850.014.24557826
GGAGGTC353700.014.2075724
ATAGGGG259950.014.18125838
GAGCTTA261350.014.10775444
TGGCATA265900.013.80930934
TATCCTA108300.013.5214825
CATAGGG288900.012.70513837
TAGCGTA9100.012.61251537
ATCCTAG123450.012.4270026
TGTATCC128500.012.1661583
GCATAGG307800.011.91768636
GGTCACT413050.011.8829887
GTCACTC419650.011.8140258
ACTCTCC420750.011.79511911
GTATCCT128750.011.7406954
TGGGAGG334700.011.7342882
TCAGACG98250.011.6076322
Produced by FastQC (version 0.11.5)