FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852805

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852805
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47527989
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCATAG151300.011.53748145
GCGTAGG150450.011.34921136
CGTAGGG151300.011.34516537
GGCGTAG151950.011.25220535
TCAGACG69450.011.0216562
GGGAGGT248450.010.6654133
TGGCGTA163150.010.53462634
GTAGGGG165550.010.43652338
GTTGTCG22150.010.05530726
AGGGATC126750.09.9822532
ACCCGGC73250.09.925775
CGTGGCG116200.09.8035771
CGTCGAT20450.09.79408912
GGAGGTC254000.09.5985514
GAGCATA191250.09.45730744
TCACCCG82300.09.3007773
CACCGAA250400.09.27114945
ACTCTCC251750.09.21638611
ATAGCTC71350.09.20673939
GTGGCGA125050.09.0737552
Produced by FastQC (version 0.11.5)