FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852807

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852807
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41861729
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCA3251560.7767381036745998No Hit
CTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA904200.21599681178959426No Hit
CCTGGCTTCTGTGAGCACCGTGCTGACCTCCAAATACCGTTAAGCTGGAGC897250.214336584138701No Hit
CTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCACCTCCC843990.20161374605430177No Hit
CAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAAC762620.18217594404664939No Hit
CAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCC642080.1533811467748979No Hit
CCAAGACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTTA637570.1523037904143902No Hit
CACCTCCCCGCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGACAAGTTC604550.14441591746007434No Hit
CAAGTTCCTGGCTTCTGTGAGCACCGTGCTGACCTCCAAATACCGTTAAGC557870.13326492080630498No Hit
GTTCCTGGCTTCTGTGAGCACCGTGCTGACCTCCAAATACCGTTAAGCTGG555860.13278476863676605No Hit
CGCCTCCCTGGACAAGTTCCTGGCTTCTGTGAGCACCGTGCTGACCTCCAA485950.11608455064051464No Hit
CACAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACT461970.11035616804074193No Hit
CTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCCTGA460950.11011250873082666No Hit
GTCAACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCAC459240.10970402106420402No Hit
AGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCC454350.10853588966666906No Hit
CCCACCTCCCCGCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGACAAGT448980.10725309506446808No Hit
CTTTGGCAAAGAATTCACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGT430220.10277167481543824No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACAGAC610050.029.215731
ACAGACT606900.029.0006712
AGACTCA694950.025.4270614
CAGACTC702800.025.1617473
GACTCAG711400.024.8677465
ACTCAGA726650.024.2620686
CTCAGAG883000.019.8208087
TCAGAGA892600.019.7616218
AACCCAC914200.019.21672616
AGAACCC918850.019.19789114
GAGAACC958850.018.53070313
ACCCACC953800.018.45451417
CAGAGAG961150.018.425099
GAACCCA963500.018.33632515
AGAGAAC999750.017.83119212
AGAGAGA1068600.016.80845510
CCGACAA1039950.016.74127240
GCCGACA1039650.016.68986139
TGCCGAC1048600.016.64635738
CGACAAG1068850.016.33491941
Produced by FastQC (version 0.11.5)