FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852812

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852812
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49992647
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1030030.20603629969823362No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG535130.10704174155851359No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC210150.025.5330094
ACCGCGT219600.024.3420476
GACCGCG219450.024.338185
CAGACCG223900.024.0654743
CCGCGTT235300.022.7178637
CGCGTTC242200.022.0799478
GAACGAT240100.021.99360845
TCAGACC274000.020.453432
AATACGG263800.020.43640732
ATACGGA270800.019.9829233
TACGGAG270000.019.89200634
AGAACGA274750.019.35895344
CTCAGAC322100.019.2231221
CAATACG290400.018.72722631
GCGTTCT292350.018.400079
ACGGAGA299300.018.03486835
AAGAACG313350.017.33344543
CGGAGAG316400.016.98859236
CCCAATA325350.016.90267829
ACGCTAC186500.016.20288324
Produced by FastQC (version 0.11.5)