FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852815

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852815
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45744416
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGGC127150.017.4968325
ACGGACA141700.015.50895318
TCACCCG149550.015.4316283
CACCCGG148600.015.2133864
CGGACAC146650.015.10799913
CGGACAG146850.014.71995619
CCGGACA150550.014.71688212
AGCTTAG100600.014.21927645
ATAGCTC147250.014.1839839
GGCATAG100250.014.16010635
CATAGGG104000.013.62645437
CACGGAC164100.013.59739117
GCCCGGA166400.013.51755110
ACACGGA164450.013.33629216
TAGCTCT161000.012.9023940
GACACGG171350.012.79927515
GCATAGG111850.012.71075436
TTGATAG175300.012.59569436
TGATAGC173900.012.51535337
GAGCTTA116700.012.31597344
Produced by FastQC (version 0.11.5)