FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852817

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852817
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51774195
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4135490.7987550554866184No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG119700.013.08500445
ACCCGGC87550.012.284715
GTTGTCG27350.011.26939426
ATAGCTC95350.010.7592939
TCAGACG123650.010.7010482
CACCGAA233000.010.54691745
GGCGTAG65150.010.53225838
GGCATAG146850.010.51051235
CGTAGGG65100.010.50648540
GCGTAGG64650.010.47459939
CGGACAC100100.010.45275413
GAGCTTA151250.010.41507244
CCGGACA102650.010.36823112
TCACCCG113250.010.1736743
ACGGACA108700.09.99706818
GCACCGA244800.09.94661944
CACCCGG115800.09.7538314
CATAGGG158100.09.73350337
TAGCTCT105500.09.72472340
TAGCGTA10650.09.71746937
Produced by FastQC (version 0.11.5)