FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852820

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852820
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50139653
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG921670.18382057809614277No Hit
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG568540.11339129131986614No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT548150.10932464969392588No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC122150.023.4855144
CAGACCG127650.022.6674943
CTACACG116050.017.41038945
CCGTACG91400.015.9272744
CTCAGAC232400.015.640031
CGTACGC93850.015.5354445
TCACGCT365050.015.4332258
AACGCTA353850.015.24771223
ACGCTAC356150.015.11765524
GTCCGTT36950.014.9837951
GCCGGCT340500.014.977661
TCAGACC201850.014.8812022
CGCTACC363600.014.7460225
AAAACGC368900.014.66224821
GACCGCG198550.014.6411625
TTTCGCC123100.014.53060713
TTCGACG18150.014.503876
CGGATAC112150.014.4850923
CCCGGAT173100.014.4580231
CGTGGTT363600.014.442924541
Produced by FastQC (version 0.11.5)