FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852821

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852821
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50139653
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA867710.17305863684377712No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG685300.1366782494485951No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG671910.1340077084298928No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG201450.016.8303545
GGCATAG208200.016.3496535
ATAGGGG220550.015.36255338
GGTCACT294950.015.3262047
GAGCTTA225550.015.181499544
AGGTCAC303800.015.1776926
GTCACTC301050.015.1715418
GGGAGGT343500.014.3329383
ACTCTCC323350.014.25712111
GGAGGTC331400.014.11554
GAGGTCA331400.014.094535
TCACTCT338000.013.8657989
GTTGTCG24700.013.84501326
AGGGATC164750.013.3640972
TAGCGTA8600.013.34196537
CACTCTC350250.013.1045510
TGGCATA265300.012.92402934
CATAGGG266450.012.85129837
TCACCCG83300.012.6747973
ACCCGGC83300.012.5118355
Produced by FastQC (version 0.11.5)