FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852822

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852822
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49363331
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1437360.29117970179119396No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG776610.15732528260704287No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG49200.029.177176
TCGACGA59150.024.6872757
GTTCGAC60650.023.965395
CGGTATA76200.023.7991820
CGACGAG62350.023.0232948
GAACGAT330600.022.07093445
AGACCGC340350.021.412534
GACCGCG338850.021.3413165
ACCGCGT340100.021.316046
CAGACCG351850.020.814783
CCGCGTT353350.020.5356227
CGCGTTC361500.020.047758
TCAGACC389600.019.2946052
AGTTCGA81300.018.4317464
AGAACGA399800.018.32391444
AATACGG400900.018.15566832
ATACGGA402100.018.10707933
GCGTTCT403950.017.9911169
TACGGAG401750.017.98284534
ATGACGG98300.017.4869416
Produced by FastQC (version 0.11.5)