FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852824

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852824
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45635417
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT601650.13183839209796197No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT146350.021.6261836
AGACCGC151650.020.884794
CAGACCG158000.020.330463
CCGCGTT157200.020.1478887
GAACGAT154950.020.08288845
GACCGCG159750.019.8544645
CGCGTTC162200.019.5545448
AACGCTA137400.017.63681823
ACGCTAC136350.017.60867724
TCAGACC190800.017.0948852
AGAACGA186250.016.91557744
ATACGGA184550.016.83980433
AATACGG186000.016.6845532
CGCTACC142600.016.53952425
TACGGAG187800.016.45293234
GCGTTCT195950.016.3245749
CAATACG195050.015.94581231
AAGAACG200750.015.59359143
TTCACGC166050.015.4570357
AAAACGC158450.015.40398521
Produced by FastQC (version 0.11.5)