FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852825

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852825
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45635417
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG507100.11111983484231118No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG175550.015.54900445
GGGAGGT253150.015.100863
GGCATAG185300.014.70407935
ATAGGGG187350.014.60272238
GGAGGTC268100.014.3338174
TGGCATA203200.013.53008534
GAGCTTA205000.013.41487344
CATAGGG219600.012.47923337
ACTCTCC315250.012.18590711
TGGGAGG246050.011.9126572
GCATAGG230900.011.88838936
TAGGGGA353250.010.53506239
ATAACGC80550.010.44428239
GTTGTCG24700.010.38322626
GTCAGGA163050.010.3454571
TGTCCAA372750.010.29037526
CTGGGAG331300.010.2373141
GCTCTGT369550.010.23199722
CACCCGG89200.010.0218711
TAACGCC84900.09.9895140
Produced by FastQC (version 0.11.5)