FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852827

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852827
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46473746
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG137300.015.34062445
GGCATAG140800.014.7072735
ATAGGGG146500.014.31846938
GAGCTTA155450.013.60801144
CATAGGG160000.012.95512437
TGGCATA170550.012.43393734
GCATAGG168650.012.30437836
GGGAGGT255750.012.1336913
AGCGCTC68950.011.8296489
GGAGGTC251600.011.7807624
ACTCTCC248200.011.60625811
TCAGACG76250.010.9624632
TAGGGGA257000.010.7044739
TAGCGTA6550.010.66336137
GTTGTCG16700.010.51376326
ATAGCTC60300.010.42484239
CGCTCTC79750.010.05873911
CAGCGCT89600.09.5797648
ACGGACA71800.09.57649818
TCTCCCC307600.09.40886813
Produced by FastQC (version 0.11.5)